Protocols

Working Protocols are linked below

Animal Work

Mouse Synovial Fluid Isolation

Near Infrared In Vivo Imaging

Manual von Frey Testing

Randall-Selito Analgesiometer Testing

Whole-Joint Dissection

Knee Joint Homogenization

Tissue Homogenization for RNA Isolation

Mouse Knee Synovial Dissection

Cell Culture

Cell Culture Media

Feeding, Counting, and Passaging Cells

Human FLS - Cytokine and Cannabinoid Treatment

Bone Marrow Monocyte Isolation and Differentiation

Fixing Adherent Cells

Alamar Blue

Mouse Knee FLS Isolation

FLS Cytokine Experimental Protocol

Histology

Whole Knee Joint Dissection and Fixation

Decalcification Methods

Fixation, Decalcification, and Cryoprotection

Submitting for Paraffin Processing

Paraffin Embedding

General Formalin Fixed, Paraffin-Embedded IHC

General Frozen Section IHC Protocol

RNA Isolation and PCR

RNA Isolation

Nanodrop Quantification

Procellys Homogenization of Synovial Tissue

RNA Clean-Up

RT-PCR

Reagents

Protein Lysis Buffer Recipe